Human VEGF ELISA Kit
Human VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human VEGF-A.
Product Specifications
Product Name Alternative
VEGFA; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor
Reactivity
Human
Field of Research
Cardiovascular Research; Cell Biology
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
16 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
31.25 pg/mL-2000 pg/mL
Available Sizes
Frequently Asked Questions
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