Human TIMP-1 ELISA Kit

Human TIMP-1 ELISA Kit employs a two-site sandwich ELISA to quantitate Human TIMP-1.

Product Specifications

Product Name Alternative

TIMP1; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity; fibroblast collagenase inhibitor; Metalloproteinase inhibitor 1

Reactivity

Human

Field of Research

Cancer Biology

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Sensitivity

9.4 pg/mL

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Notes

For research use only.

Applications Notes

Human TIMP-1 ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP-1 in samples. An antibody specific for TIMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-1 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.