Human MMP-9 ELISA Kit
Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate Human MMP-9.
Product Specifications
Product Name Alternative
MMP 9; MMP-9; MMP9; Matrix Metalloproteinase 9; GELB; Type V collagenase; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Reactivity
Human
Field of Research
Cancer Biology; Musculoskeletal & Connective Tissue Research
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
16 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate MMP-9 in samples. An antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
31.25 pg/mL- 2000 pg/mL
Available Sizes
Frequently Asked Questions
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