Human IL-23 ELISA Kit

IL23; IL 23; IL 23A; IL-23 subunit alpha; IL-23-A; IL-23p19; Il23a; IL23P19; Interleukin 23 alpha subunit p19; Interleukin-23 subunit alpha; Interleukin-23 subunit p19

Human IL-23 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-23 in samples. An antibody specific for IL-23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-23 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-23 bound in the initial step. The color development is stopped and the intensity of the color is measured.