Human IL-18 ELISA Kit
Human IL-18 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-18.
Product Specifications
Product Name Alternative
IL-18; IL18; IL 18; IFN gamma inducing factor; Interleukin 18; Interleukin-18; Interleukin 18 (interferon-gamma-inducing factor)
Reactivity
Human
Field of Research
Immunology & Inflammation
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
40 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human IL-18 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-18 in samples. An antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
156 pg/mL- 10000 pg/mL
Available Sizes
Frequently Asked Questions
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