Human IL-18 ELISA Kit

Human IL-18 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-18.

Product Specifications

Product Name Alternative

IL-18; IL18; IL 18; IFN gamma inducing factor; Interleukin 18; Interleukin-18; Interleukin 18 (interferon-gamma-inducing factor)

Reactivity

Human

Field of Research

Immunology & Inflammation

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Sensitivity

40 pg/mL

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Notes

For research use only.

Applications Notes

Human IL-18 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-18 in samples. An antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound in the initial step. The color development is stopped and the intensity of the color is measured.