Human IL-6 ELISA Kit
Human IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-6.
Product Specifications
Product Name Alternative
IL 6; IL6; BSF2; HGF; HSF; IFNB2; IL-6; B cell stimulatory factor-2; B-cell differentiation factor; CTL differentiation factor; OTTHUMP00000158544; hybridoma growth factor; interleukin 6; interleukin BSF-2
Reactivity
Human
Field of Research
Immunology & Inflammation
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
2 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-6 in samples. An antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
3.125 pg/ml-200 pg/ml
Available Sizes
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