Human G-CSF ELISA Kit

C17orf33; Colony stimulating factor 3 (granulocyte) ; CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2

Human G-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate G-CSF in samples. An antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G-CSF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.