Human Amyloid Beta Peptide 40 Antibody (Aβ40-Ab) ELISA Kit

This ELISA kit uses the Double Antigen Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Aβ40 .Samples (or Standards) are added to the micro ELISA plate wells and combined with antigen. Then a biotinylated detection antigen and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ40-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human Aβ40-Ab. You can calculate the concentration of Human Aβ40-Ab in the samples by comparing the OD of the samples to the standard curve.