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Acylglycerol kinase Antibody

Goat polyclonal antibody to AGK

Product Specifications

Product Name Alternative

Anti acylglycerol kinase antibody, anti FLJ10842 antibody, anti MULK antibody, anti multi-substrate lipid kinase antibody, anti multiple substrate lipid kinase antibody, anti AGK antibody

Reactivity

Human

Target

Acylglycerol kinase

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CDPRKREQMLTSP

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml

Molecular Weight

47.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1kDa according to NP_060708.1) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

55750

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