Insulysin/Insulinase Antibody
Goat polyclonal antibody to IDE
Product Specifications
Product Name Alternative
Anti insulinase antibody, anti insulysin antibody, anti insulin-degrading enzyme antibody, anti FLJ35968 antibody, anti INSULYSIN antibody, anti IDE antibody
Reactivity
Human
Target
Insulysin / Insulinase
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QQYNFDRDNTE
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
118, 54
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1) . In transfected HEK293 transiently expressing Insulysin a band of approx. 110kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.
Prediction Reactivity
Canine
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
3416
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