FS-Human CCL5/RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

CCL5, chemokine (C-C motif) ligand 5, D17S136Enormally T-expressed, and presumably secreted, EoCP, Eosinophil chemotactic cytokine, RANTES, SISd, SIS-delta, small inducible cytokine A5 (RANTES), small inducible cytokine subfamily A (Cys-Cys), member 5, Small-inducible cytokine A5, T cell-specific protein P228, T-cell specific protein p288, TCP228T-cell-specific protein RANTES

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCL5/RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL5/RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL5/RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CCL5/RANTES. You can calculate the concentration of Human CCL5/RANTES in the samples by comparing the OD of the samples to the standard curve.