MIC1/C18orf8 Antibody
Goat polyclonal antibody to C18orf8
Product Specifications
Product Name Alternative
Anti C18orf8 antibody, anti chromosome 18 open reading frame 8 antibody, anti FLJ23453 antibody, anti HsT2591 antibody, anti MIC1 antibody, anti colon cancer associated protein Mic1 antibody, anti colon cancer-associated protein Mic1 antibody
Reactivity
Human
Target
MIC1 / C18orf8
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DKLNHEYKKYLDAEQ
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-2=10μg/ml
Molecular Weight
75
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 90kDa band in Human Bone Marrow, Spleen and Tonsil lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 75kDa according to NP_037458.3. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
29919
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