Atoh7 (mouse) Antibody
Goat polyclonal to Atoh7
Product Specifications
Product Name Alternative
Anti Atoh7 antibody, anti atonal homolog 7 (Drosophila) antibody, anti Math antibody, anti atonal homolog 7 antibody
Target
Atoh7 (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CEQRGRDHPYLP
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.3 μg/ml
Molecular Weight
16.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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