Fascin 2 Antibody
Goat polyclonal antibody to FSCN2
Product Specifications
Product Name Alternative
Anti fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) antibody, anti RFSN antibody, anti RP30 antibody, anti fascin 2 antibody, anti retinal fascin antibody
Reactivity
Mouse
Target
Fascin 2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CHHRGSNQLDTNR
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, IHC-P: 4 μg/ml
Molecular Weight
57.4; 55.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 75kDa and 22kDa in Mouse Eye lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 57.4kDa according to NP_001070650.1 and 55.1kDa according to NP_036550.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Human, Rat
Tested Applications
ELISA, ICC, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
25794
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