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GLuR5/GRIK1 Antibody

Goat polyclonal antibody to GRIK1

Product Specifications

Product Name Alternative

Anti GLUR5 antibody, anti GRIK1 antibody, anti glutamate receptor, ionotropic, kainate 1 antibody, anti EAA3 antibody, anti EEA3 antibody, anti GLR5 antibody, anti excitatory amino acid receptor 3 antibody

Reactivity

Human

Target

GLuR5 / GRIK1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QCKQTHPTNSTS

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

104; 103

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24 (41) :8986-93.; PMID: 15483117) . Recommended concentration: 1-3 μg/ml. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

2897

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