GLuR5/GRIK1 Antibody
Goat polyclonal antibody to GRIK1
Product Specifications
Product Name Alternative
Anti GLUR5 antibody, anti GRIK1 antibody, anti glutamate receptor, ionotropic, kainate 1 antibody, anti EAA3 antibody, anti EEA3 antibody, anti GLR5 antibody, anti excitatory amino acid receptor 3 antibody
Reactivity
Human
Target
GLuR5 / GRIK1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QCKQTHPTNSTS
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
104; 103
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24 (41) :8986-93.; PMID: 15483117) . Recommended concentration: 1-3 μg/ml. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
2897
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