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HTATSF1 Antibody

Goat polyclonal antibody to HTATSF1

Product Specifications

Product Name Alternative

Anti HTATSF1 antibody, anti HIV-1 Tat specific factor 1 antibody, anti RP1-196E23.2 antibody, anti TAT-SF1 antibody, anti dJ196E23.2 antibody, anti HIV TAT specific factor 1 antibody, anti cofactor required for Tat activation of HIV-1 transcription antibody

Reactivity

Human

Target

HTATSF1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QELYGDGKDGDTQTD

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml

Molecular Weight

85.9

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274 (5287) :605-10.; PMID: 8849451) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

27336

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