HTATSF1 Antibody
Goat polyclonal antibody to HTATSF1
Product Specifications
Product Name Alternative
Anti HTATSF1 antibody, anti HIV-1 Tat specific factor 1 antibody, anti RP1-196E23.2 antibody, anti TAT-SF1 antibody, anti dJ196E23.2 antibody, anti HIV TAT specific factor 1 antibody, anti cofactor required for Tat activation of HIV-1 transcription antibody
Reactivity
Human
Target
HTATSF1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QELYGDGKDGDTQTD
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 1-3 μg/ml
Molecular Weight
85.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274 (5287) :605-10.; PMID: 8849451) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
27336
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