Robo3 (mouse) Antibody
Goat polyclonal antibody to Robo3
Product Specifications
Product Name Alternative
Anti Robo3 antibody, anti roundabout homolog 3 (Drosophila) antibody, anti Rbig1 antibody, anti RIG1 antibody, anti Rig-1 antibody, anti Robo3a antibody, anti Robo3b antibody, anti retinoblastoma inhibiting gene 1 antibody
Target
Robo3 (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RRQKQRKELSHYT
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.1 μg/ml
Molecular Weight
143
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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