Human VIP (Vasoactive Intestinal Peptide) Microsample ELISA Kit

Product Specifications

Product Name Alternative

PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

UniProt

P01282

Reactivity

Human

Field of Research

Gastroenterology & Hepatology, Metabolism Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

2.65 pg/mL

Notes

For research use only.

Applications Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VIP in the samples is then determined by comparing the OD of the samples to the standard curve.