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CLPB Antibody

Goat polyclonal antibody to CLPB

Product Specifications

Product Name Alternative

Anti CLPB antibody, anti ClpB caseinolytic peptidase B homolog (E. coli) antibody, anti FLJ13152 antibody, anti HSP78 antibody, anti SKD3 antibody, anti suppressor of potassium transport defect 3 antibody

Target

CLPB

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DKDSKTRRLDIRAP

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.1 μg/ml

Molecular Weight

78.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

81570

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