What is the catalog number for DIO2 Antibody?

The catalog number for DIO2 Antibody is orb19555.

What sizes are available for DIO2 Antibody?

DIO2 Antibody is available in the following size(s): 100 µg.

Is DIO2 Antibody in stock?

DIO2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

DIO2 Antibody

Goat polyclonal antibody to DIO2

Product Specifications

Product Name Alternative

Anti DIO2 antibody, anti deiodinase, iodothyronine, type II antibody, anti 5DII antibody, anti D2 antibody, anti SelY antibody, anti TXDI2 antibody, anti thyroxine deiodinase, type II antibody, anti type 2 iodothyronine deiodinase antibody, anti type II iodothyronine deiodinaseN: type-II 5\1deiodinase antibody

Reactivity

Human, Mouse

Target

DIO2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EVKKHQNQEDRC

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, IHC-P: 2-3 μg/ml

Molecular Weight

30.5; 34.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3ug/ml. Data obtained from anonymous customer.WB: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5kDa according to NP_NP_000784.2 and of 34.6kDa according to NP_001007024.1. The 65-70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Porcine, Rat

Tested Applications

ELISA, FC, IF

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.