Goat anti-Ide (mouse) Antibody
Goat polyclonal antibody to Ide
Product Specifications
Product Name Alternative
Anti Ide antibody, anti insulin-degrading enzyme antibody, anti 1300012G03Rik antibody, anti 4833415K22Rik antibody, anti AA675336 antibody, anti AI507533 antibody, anti INSULYSIN antibody, anti insulinase antibody, anti insulysin antibody
Reactivity
Mouse, Rat
Target
Ide (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QQYNYDRDNIE
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 1-3 μg/ml
Molecular Weight
118
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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