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Goat anti-Ide (mouse) Antibody

Goat polyclonal antibody to Ide

Product Specifications

Product Name Alternative

Anti Ide antibody, anti insulin-degrading enzyme antibody, anti 1300012G03Rik antibody, anti 4833415K22Rik antibody, anti AA675336 antibody, anti AI507533 antibody, anti INSULYSIN antibody, anti insulinase antibody, anti insulysin antibody

Reactivity

Mouse, Rat

Target

Ide (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QQYNYDRDNIE

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml

Molecular Weight

118

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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