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Klenow Fragment

Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.

Product Specifications

Product Name Alternative

Large Fragment of DNA Polymerase I, recombinant, E. coli

Concentration

5 units/μl

Purity

The enzyme is greater than 98% pure as indicated by SDS-

Form

Liquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol)

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Fill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends.

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