CCKA Receptor (mouse) Antibody
Goat polyclonal antibody to Cckar
Product Specifications
Product Name Alternative
Anti Cckar antibody, anti cholecystokinin A receptor antibody, anti AW106902 antibody
Reactivity
Rat
Target
CCKA Receptor (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KFDASQKKSAKEKR
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.1-0.3 μg/ml
Molecular Weight
48.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1) . Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51 (11) :1501-13; PMID: 14566022) . Recommended concentration: 0.1-0.3 μg/ml.
Prediction Reactivity
Mouse
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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