Human Tau (Tau Proteins) ELISA Kit

Włodzimierz Płotek 1, Artur Bekała 2, Jadwiga Łuczak-Wawrzyniak 2, Katarzyna Dudzińska-Rapczewska 2, Małgorzata Gasińska-Błotniak 1, Marcin Cybulski 3, Agnieszka Kubik-Komar 4, Elżbieta Kubera 4, Maciej Wilczak 5, Wojciech Dąbrowski 6 Is general anesthesia neuroinjury-free and safe for gynecological patients' cognition? A comparison of two typical anesthesia schemes based on propofol and sevoflurane Anaesthesiol Intensive Ther, (2025)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Tau. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tau. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Tau, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tau in the samples is then determined by comparing the OD of the samples to the standard curve.