Goat anti-Hap1 (mouse) Antibody
Goat polyclonal antibody to Hap1
Product Specifications
Product Name Alternative
Anti Hap1 antibody, anti huntingtin-associated protein 1 antibody, anti RP23-392I3.11, HAP-1, MGC31449 antibody
Reactivity
Mouse, Rat
Target
Hap1 (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QDAHSKRQQKQK
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1-0.3 μg/ml
Molecular Weight
66.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 110kDa band in Mouse and Rat Brain lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.8kDa according to NP_034534.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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