Goat anti-NANOG (C Terminus) Antibody
Goat polyclonal antibody to NANOG
Product Specifications
Product Name Alternative
Anti NANOG antibody, anti Nanog homeobox antibody, anti homeobox transcription factor Nanog antibody, anti homeobox transcription factor Nanog-delta 48 antibody
Reactivity
Human
Target
NANOG (C Terminus)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DLFLNYSMNMQPED
Field of Research
Stem Cell & Developmental Biology; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.05-0.1μg/ml, IHC-P: 1-2μg/ml
Molecular Weight
34.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.WB: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1) . Recommended concentration: 0.05-0.1 μg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
79923
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