CHD5 Antibody
Goat polyclonal antibody to CHD5
Product Specifications
Product Name Alternative
Anti CHD5 antibody, anti chromodomain helicase DNA binding protein 5 antibody, anti DKFZp434N231 antibody, anti KIAA0444 antibody
Reactivity
Human
Target
CHD5
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QSRRQLKSDRDKP
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, IF/ICC: 10μg/ml, FACS: 10ug/ml
Molecular Weight
223
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 90kDa band in Human Brain (Cerebellum and Frontal Cortex) and Adrenal Gland lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 223kDa according to NP_056372.1. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, FC, IF
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
26038
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