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FRAG1 (mouse) Antibody

Goat polyclonal antibody to Atad5

Product Specifications

Product Name Alternative

Anti Atad5 antibody, anti ATPase family, AAA domain containing 5 antibody, anti C17orf41 antibody, anti chromosome 17 open reading frame 41 antibody, anti FLJ12735 antibody, anti FRAG1 antibody, anti chromosome fragility associated gene 1 antibody, anti hypothetical protein LOC237877 antibody, anti RP23-328O2.1 antibody, anti C130052G03Rik antibody

Target

FRAG1 (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QSKDQPLRKSQK

Field of Research

Cancer, DNA Damage, Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.01 μg/ml

Molecular Weight

204

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 60kDa band in lysates of cell lines NIH3T3 and NSO after 0.01 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 204kDa according to NP_001025027.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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