Mouse Surfactant Associated Protein A (SPA) ELISA Kit
Mouse Surfactant Associated Protein A (SPA) ELISA Kit
Product Specifications
Product Name Alternative
PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein
UniProt
P35242
Reactivity
Mouse
Field of Research
Respiratory Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, lung lavage fluid and other biological fluids
Sensitivity
0.18 ng/mL
Notes
For research use only.
Applications Notes
Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SPA in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.78-50 ng/mL
Available Sizes
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