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STS2/TULA (internal) Antibody

Goat polyclonal antibody to UBASH3A

Product Specifications

Product Name Alternative

Anti TULA antibody, anti STS2 CBL-interacting protein 4 antibody, anti UBASH3A antibody, anti ubiquitin associated and SH3 domain containing, A antibody, anti T-cell ubiquitin ligand protein antibody, anti Suppressor of T-cell receptor signaling 2 antibody, anti CLIP4 antibody

Reactivity

Human

Target

STS2 / TULA (internal)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QQCSTPDGKYYRPD

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

74.1; 69.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

53347

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