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Rat Afamin (AFM) ELISA Kit

Rat Afamin (AFM) ELISA Kit

Product Specifications

Product Name Alternative

ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

UniProt

P36953

Reactivity

Rat

Target

AFM

Field of Research

Cancer Biology, Gastroenterology & Hepatology, Infectious Disease & Virology

Assay Type

Competitive

Assay Performance Time

2h

Sample Type

Serum, plasma and other biological fluids

Sensitivity

0.89 μg/mL

Notes

For research use only.

Applications Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve.

Tested Applications

ELISA

Dynamic Range

3.13-200 μg/mL

Available Sizes

Frequently Asked Questions

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