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APPL1 Antibody

Goat polyclonal antibody to APPL1

Product Specifications

Product Name Alternative

Anti APPL1 antibody, anti DIP13alpha antibody, anti adaptor protein containing pH domain, PTB domain and leucine zipper motif antibody, anti signaling adaptor protein DIP13alpha antibody

Target

APPL1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NRYSRLSKKRENDK

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.3-1 μg/ml

Molecular Weight

79.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 80kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2) . Calculated MW of 79.7kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1 μg/ml.

Prediction Reactivity

Bovine, Canine, Human, Mouse, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

26060

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