APOM Antibody
Goat polyclonal antibody to APOM
Product Specifications
Product Name Alternative
Anti APOM antibody, anti apolipoprotein M antibody, anti G3a antibody, anti HSPC336 antibody, anti MGC22400 antibody, anti NG20 antibody, anti NG20-like protein antibody, anti G3a antibody, anti NG20 antibody
Reactivity
Human
Target
APOM
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
PRNQEACELSNN
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 0.2 μg/ml, IHC-P: 3-6μg/ml
Molecular Weight
21.3
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6ug/ml.WB: Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
55937
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