Zebrafish Malondialdehyde (MDA) ELISA Kit

Adam Bownik, Barbara Pawlik-Skowrońska Effects of cyanobacterial metabolites and their mixtures on biomarkers of oxidative stress in RTgill-W1 cells Toxicol In Vitro, 112, 106195 (2026)

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish MDA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish MDA in the samples is then determined by comparing the OD of the samples to the standard curve.