MBD2 (isoform 1) Antibody
Goat polyclonal antibody to MBD2
Product Specifications
Product Name Alternative
Anti MBD2 antibody, anti methyl-CpG binding domain protein 2 antibody, anti DKFZp586O0821 antibody, anti DMTase antibody, anti NY-CO-41 antibody
Reactivity
Human
Target
MBD2 (isoform 1)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RNDPLNQNKGKPDLN
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
43.3; 31.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml.WB: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26 (19) :7224-35.; PMID: 16980624) . Recommended concentration: 0.3-1 μg/ml.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
8932
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