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MBD2 (isoform 1) Antibody

Goat polyclonal antibody to MBD2

Product Specifications

Product Name Alternative

Anti MBD2 antibody, anti methyl-CpG binding domain protein 2 antibody, anti DKFZp586O0821 antibody, anti DMTase antibody, anti NY-CO-41 antibody

Reactivity

Human

Target

MBD2 (isoform 1)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RNDPLNQNKGKPDLN

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

43.3; 31.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml.WB: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26 (19) :7224-35.; PMID: 16980624) . Recommended concentration: 0.3-1 μg/ml.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

8932

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