Semaphorin 3E (internal) Antibody

Goat polyclonal antibody to SEMA3E

Product Specifications

Product Name Alternative

Anti SEMA3E antibody, anti sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody, anti KIAA0331 antibody, anti M-SEMAH antibody, anti M-SemaK antibody, anti M-sema H antibody, anti SEMAH antibody, anti coll-5 antibody, anti sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E antibody, anti semaphorin 3E antibody

Reactivity

Human

Target

Semaphorin 3E (internal)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NGGRYGTTKDYPDD

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.03 μg/ml, IHC-P: 4-6μg/ml

Molecular Weight

89.2; 82.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.