Mouse Gremlin 2 (GREM2) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GREM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
Cysteine knot superfamily 1, BMP antagonist 2; Protein related to DAN and cerberus; PRDC; Cktsf1b2
UniProt
O88273
Reactivity
Mouse
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
12.2 pg/mL
Notes
For research use only.
Applications Notes
Dynamic Range
31.25-2000 pg/mL
Available Sizes
Frequently Asked Questions
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