Scicons Double-stranded RNA (dsRNA) ELISA kit (J2 based)

Upon receipt, store entire kit at -20°C. Once the kit is thawed, you may keep it at 4°C for 5 days. For long-term storage, it is recommended to aliquot and freeze the antibody and dsRNA components at –20°C.

We recommend using the kit to detect viral dsRNAs or large natural or synthetic dsRNAs of non-viral origin in nucleic acid extracts, as well as to detect the presence of undesired dsRNA molecules in artificially synthesized (m) RNA preparations. Serial dilutions of the Poly (I:C) dsRNA standard (included in the kit) can be used as a positive control. For the exact detection protocol we refer to the kit manual that can be downloaded from our website. Caution: The Poly (I:C) dsRNA positive control included in this sandwich ELISA kit is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies J2 and/or K2 used in this kit may exhibit a different degree of reactivity with different dsRNAs obtained from synthetic or natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out for the OD450.