PIK3CA/P110alpha Antibody

Goat polyclonal antibody to PIK3CA

Product Specifications

Product Name Alternative

Anti PIK3CA antibody, anti p110-alpha antibody, anti phosphoinositide-3-kinase, catalytic, alpha polypeptide antibody, anti MGC142161 antibody, anti MGC142163 antibody, anti PI3K antibody, anti PI3-kinase p110 subunit alpha antibody, anti PtdIns-3-kinase p110 antibody, anti phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha antibody, anti phosphatidylinositol 3-kinase, catalytic, alpha polypeptide antibody, anti phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform antibody

Reactivity

Human

Target

PIK3CA / P110alpha

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HQWLKDKNKGEIYD

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 1 μg/ml) ., IHC-P: 5 μg/ml

Molecular Weight

124

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3ug/ml.Immunohistochemistry data: see also publciation Abubaker J et al. Mol Cancer. 2009 Jul 28;8:51. (PMID: 19638206) .WB: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/ml) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.