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PRPF31 Antibody

Goat polyclonal antibody to PRPF31

Product Specifications

Product Name Alternative

Anti PRPF31 antibody, anti PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) antibody, anti DKFZp566J153 antibody, anti NY-BR-99 antibody, anti PRP31 antibody, anti RP11 antibody, anti PRP31 pre-mRNA processing factor 31 homolog (yeast) antibody, anti pre-mRNA processing factor 31 homolog antibody, anti pre-mRNA processing factor 31 homolog (yeast) antibody

Reactivity

Human

Target

PRPF31

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KELGNSLDKCKNNEN

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 1-3 μg/ml

Molecular Weight

55.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 60kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 55.4kDa according to NP_056444.2) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, FC, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

26121

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