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ICAM5 precursor Antibody

Goat polyclonal antibody to ICAM5

Product Specifications

Product Name Alternative

Anti ICAM5 antibody, anti intercellular adhesion molecule 5, telencephalin antibody, anti TLCN antibody, anti TLN antibody, anti intercellular adhesion molecule 5 antibody, anti telencephalin antibody

Reactivity

Human

Target

ICAM5 precursor

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ESPAEGEVFAIQ

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.05 μg/ml, IHC-P: 2-4μg/ml

Molecular Weight

97.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

7087

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