Human Melanoma-associated antigen D2 (MAGED2) ELISA Kit

11B6 , BCG 1 , BCG-1 , Breast cancer-associated gene 1 protein , HCA10 , Hepatocellular carcinoma-associated protein JCL-1 , JCL 1 , MAGD2 , MAGE D , MAGE D2 antigen , MAGE-D2 antigen , MAGED2 , Melanoma antigen family D 2 , Melanoma associated antigen D2 , Melanoma-associated antigen D2

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAGED2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAGED2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAGED2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAGED2. You can calculate the concentration of Human MAGED2 in the samples by comparing the OD of the samples to the standard curve.