Human Renalase (RNLS) ELISA Kit

65344N21Rik, AI452315, AW644, C1orf59, Chromosome 1 open reading frame 59, FLJ11218, HGNC:25641, Hypothetical protein LOC55328, MAO C, MAO-C, mMAO C, Monoamine oxidase C, Monoamine oxidase-C, Renalase, Renalase FAD dependent amine oxidase, RNLS

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RNLS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RNLS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RNLS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RNLS. You can calculate the concentration of Human RNLS in the samples by comparing the OD of the samples to the standard curve.