Robo1/DUTT1 (mouse) Antibody
Goat polyclonal antibody to Robo1
Product Specifications
Product Name Alternative
Anti Robo1 antibody, anti roundabout homolog 1 (Drosophila) antibody, anti AW494633 antibody, anti AW742721 antibody, anti DUTT1 antibody, anti roundabout homolog 1 antibody
Reactivity
Mouse
Target
Robo1 / DUTT1 (mouse)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QKARPAKKQKHQ
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
176
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 80kDa and 40kDa in lysates of Mouse Brain and Rat Brain after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 176kDa according to NP_062286.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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