VPS35 ELISA Kit

FLJ10752, MEM3, PARK17, TCCCTA00141, VPS35 retromer complex component, Vacuolar protein sorting-associated protein 35, maternal-embryonic 3, vesicle protein sortin 35

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 μL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 μL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at 37°C for 1 hour shaking at 600rpm. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 μL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes, shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 μL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 μL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.