SR-FLIVO In vivo Poly Caspase Assay
A non-cytotoxic, cell-permeant fluorescent inhibitor of caspases optimized for use in whole live animals. The probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD), coupled to a SR dye and a FMK reactive entity.
Product Specifications
Field of Research
Cell Biology
Sample Type
Whole live animal, excised tissue
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare samples and controls. 2. Dilute 10X Injection Buffer 1:10 with 45 mL diH20. 3. Reconstitute SR-FLIVO with 50 μL DMSO. 4. Dilute SR-FLIVO 1:12 with 550 μL 1X Injection Buffer. 5. Inject 100 μL intravenously. 6. Let SR-FLIVO circulate 30-60 minutes. 7. View live tumor through a window chamber using a fluorescence microscope. 8. If not viewing directly, excise tissue. 9. If desired, label with additional stains, such as Hoechst 33342, or an antibody. 10. If desired, fix cells. 11. Analyze with a fluorescence microscope, flow cytometer, or a window chamber system. SR-FLIVO excites at 550-580 nm and emits at 590-600 nm.
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