GPX4 (Isoform a and c) Antibody
Goat polyclonal antibody to GPX4
Product Specifications
Product Name Alternative
Anti GPX4 antibody, anti glutathione peroxidase 4 (phospholipid hydroperoxidase) antibody, anti HGNC:4556 antibody, anti PHGPx antibody, anti snGPx antibody, anti glutathione peroxidase 4 antibody, anti phospholipid hydroperoxidase antibody, anti sperm nucleus glutathione peroxidase antibody
Reactivity
Human
Target
GPX4 (Isoform a and c)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EEPLVIEKDLPHY
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 0.3 μg/ml
Molecular Weight
22.1; 27.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3ug/ml.WB: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2) . Recommended concentration: 0.1-0.3 μg/ml.
Prediction Reactivity
Bovine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC, IP, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
2879
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